Indexing

Abstract:The Citrus reticulata is a industrially important horticultural plant of the family Rutaceae. The present report developed the protocol for callus induction and callus regeneration. The explants were cultured on medium containing M.S. basal medium with various concentrations of NAA. For callus induction. The treatment containing M.S. basal with 1.0 mg/l NAA were best for callus induction and proliferation. The best callus regeneration on M.S. with 1.0 mg/l BAP. The regenerated shoots were successfully rooted on M.S. with 0.5 mg/l NAA
Keywords:Citrus reticulata, Callus, multiplication

Abstract:Wound healing could be disfiguring, prolonged, and disappointing. The management of scar could be as simple as leave it alone, to surgical excision. The use of negative pressure in wound management proved to reduce the wound epidermal thickening for a transient period. This pilot study was performed to observe the difference in time and features of wound healing in albino mice with and without negative pressure, and also to perform histological analysis to the wound and report the changes, if found, at the cellular and extracellular level. Intermittent negative pressure (INP) application to the wound has been tried in this experiment to estimate its effects in mice skin with a recent wound. One albino mouse of the genus Mus musculus was purposefully wounded on abdominal wall. Two wounds were performed on the mouse; one as a test and the other was a control. The test wound was exposed to manually operated INP sessions ten minutes daily for two weeks.  Wound healing was delayed by 48 hours in the test scar, as compared to control. The test histology showed signs of an inflammatory reaction and epithelial distortion. This study concluded that applying intermittent negative pressure in mice wounds delays the wounds healing and causes an inflammatory-like response.
Keywords:Negative pressure, wound, experimental mice.
Title:The effect of intermittent negative pressure in mice wound healing; a pilot study
Author:Osama S. Abbadi, Khalid M. Nafie
ISSN 2349-7823
International Journal of Recent Research in Life Sciences (IJRRLS)
Paper Publications

Abstract:Nigeria has several culinary herbs and spices with medicinal, pharmaceutical and industrial uses. This paper presents the importance of selected culinary herbs and spices for promotion of health and wellness in Nigeria, such as: ginger (Zingiber officinale),  garlic (Allium sativum), black pepper (Piper Nigrum), African nutmeg (Monodora myristica), alligator pepper (Aframomum melegueta), negro pepper (Xylopia aethiopica), aidan tree (Tetrapleura tetraptera), African basil (Ocimum gratissimum), African locust bean (Parkia biglobosa), amaranth globe (Gongronema latifolium Benth), and turmeric (Curcuma longa). Culinary herbs and spices in Nigeria contain beneficial phytochemicals with great potentials as functional foods and nutraceuticals. They possess cholesterol-lowering and antidiabetic effects, as well as antimicrobial, antimycobacterial, antifungal, antioxidant, antibacterial, antibiotic, anti-inflammatory and antihepatotoxicity properties for treatment of diseases and for promotion of health and wellbeing of Nigerians.
Title:Culinary Herbs and Spices for Promotion of Health and Wellness in Nigeria

Abstract:About fifty percent of patients with long standing diabetes mellitus eventually develop the diabetic foot. Millions of diabetic patients are amputated annually at different levels on their feet. The diabetic foot develops either due to neuropathy or micro-vascular diseases. Diabetic foot management includes controlling the diabetes mellitus-(blood glucose level), the choice of a proper foot wear, care of the wound, and, if needed, surgical revascularization or amputation. Negative pressure wound therapy, using NPWT machines, proved its efficacy in draining and closing diabetic foot ulcers. The objective of the study was to determine the effects of applying intermittent negative pressure (INP) in patients with diabetic foot, with and without a preformed ulcer. Six non- ulcer diabetic foot patients were subjected to INP using a foot-shaped plastic cast for ten minutes every two days. Another six patients with wounded diabetic foot were subjected to INP by a traditional cupping kit for five to seven minutes every two days.  The study showed that four of the non-ulcer patients-(representing 67%), admitted that the symptoms of diabetic foot were reduced markedly, the remaining two-(representing 33%), stated that there were no improvement in symptoms, but no patient declared to be deteriorated due to the experiment. The patients with foot ulcer had got a great benefit from the INP; their wounds did heal faster than expected. In conclusion, intermittent negative pressure reduced the symptoms of diabetic foot and fastened the healing of diabetic wounds.
Keywords:Negative pressure, Diabetic foot, Amputation.

Abstract:Staphylococcus aureus, leading causes of nosocomial bacteremia, surgical wound infection, and urinary tract infection, are becoming resistant to many and sometimes all standard therapies. Staphylococcus aureus is notorious for its ability to become resistant to antibiotics. Infections caused by antibiotic-resistant strains often occur in epidemic waves initiated by one or a few successful clones. Methicillin-resistant S. aureus (MRSA) is prominently featured during these epidemics. Historically associated with hospitals and other healthcare settings, MRSA now has emerged as a widespread cause of community infections. So-called community or community-associated MRSA spreads rapidly among healthy individuals. Outbreaks of community MRSA infections have been reported worldwide and community MRSA strains are now epidemic in the United States. There is reason for concern because MRSA often are or can readily become resistant to multiple antibiotics, thus limiting treatment options. Staphylococcus aureus is the leading cause of bacterial infections in developed countries and produces a wide spectrum of diseases, ranging from minor skin infections to fatal necrotizing pneumonia. Although S. aureus infections were historically treatable with common antibiotics, emergence of drug-resistant organisms is now a major concern. Methicillin-resistant S. aureus (MRSA) was endemic in hospitals by the late 1960s, but it appeared rapidly and unexpectedly in communities in the 1990s and is now prevalent worldwide. This Review focuses on progress made toward understanding the success of community-associated MRSA as a human pathogen, with an emphasis on genome-wide approaches and virulence determinants. New rapid surveillance methods are highlighting the importance of examining enterococcal isolates at the species level. Most enterococcal infections are caused by Enterococcus faecalis, which are more likely to express traits related to overt virulence but--for the moment--also more likely to retain sensitivity to at least one effective antibiotic. The remaining infections are mostly caused by E. faecium, a species virtually devoid of known overt pathogenic traits but more likely to be resistant to even antibiotics of last resort. Effective control of multiple-drug resistant enterococci will require 1) better understanding of the interaction between enterococci, the hospital environment, and humans, 2) prudent antibiotic use, 3) better contact isolation in hospitals and other patient care environments, and 4) improved surveillance. Equally important is renewed vigor in the search for additional drugs, accompanied by the evolution of new therapeutic paradigms less vulnerable to the cycle of drug introduction and drug resistance.
Keywords:Staphylococcus aureus, nosocomial bacteremia, Methicillin-resistant S. aureus (MRSA).