Vol 5 Issue 2 April 2018-June 2018
Anacletus, F.C., Monanu, M.O., Ugwu, G. M.
Abstract: Qualitative phytochemical composition of Limonia acidissima leaf and heamatological indices of phenylhydrazine (phz)-induced anaemic wistar rats were investigated. A total of forty two (42) wistar rats of both sexes weighing between 150-200g were used. The animals were randomly divided into seven (7) groups of six rats each. Folic acid and Vitamin B12 were used as reference drugs. Aqueous extract of Limonia acidissima leaves was administered to the rats for four (4) weeks period.Qualitative phytochemical analysis on the dry leaf samples revealed the presence of the following in low abundance: Alkaloid, Saponins, Cardiac glycoside, Steroids, Phenols, Terpenoids and Proteins while flavonoids were in high abundance. Induction of anaemia significantly (p<0.05) decreased Haemoglobin (Hb) levels, Red Blood Cell (RBC) concentration, Mean Corpuscular Volume (MCV) and White Blood Cell Count (WBC) of Phenylhydrazine-Induced non- treated rats compared to normal control. In the groups treated with aqueous extract of Limonia acidissima at the doses of 100mg/kgbw, 200mg/kgbw and 300mg/kgbw, Hb, MCV,WBC and RBC were significantly (p<0.05) elevated in the first two weeks of the investigation while a further significant (p<0.05) increase occurred in the fourth week in comparison to the Phenylhydrazine-induced anemic non- treated rats. A significant increase (p<0.05) in the haematological parameters was also observed in the groups treated with folic acid and vitamin B12 when compared to the Phenylhydrazine-induced anaemic non- treated groups at the first two weeks and the fourth week.This outcome illustrates the anti-anaemic potential of aqueous extract of the leaf of Limonia acidissima and its possession of hematopoietic properties and that at a dose of 300mg/kgbw, it has the most anti-anemic potentials.
Keywords: Anaemia, phenlyhydrazine, Limonia acidissima, Haemoglobin.
Title: Anti-Anaemic Potentials of Folic Acid, Vitamin B12 and Aqueous Extract of Limonia acidissima Leaves on Phenylhydrazine-Induced Anaemic Wistar Rats
Author: Anacletus, F.C., Monanu, M.O., Ugwu, G. M.
ISSN 2349-7823
International Journal of Recent Research in Life Sciences (IJRRLS)
Paper Publications