Vol 3 Issue 1 January 2016-March 2016
Ankit Magotra, I.D.Gupta, Archana Verma, Rani Alex, Vijay Kumar, M.R. Vineeth, Ashwani Arya, A. Sakthivel Selvan
Abstract: Bovine mastitis is a very common and multietiological disease of dairy cattle which leads to huge economic losses to the dairy industry globally. In this study, the bovine breast cancer 1, early onset gene (BRCA1) was taken as a candidate gene for mastitis resistance. Breast cancer 1 (BRCA1) is one of the genes which predispose organism to early-onset breast cancer, and is involved in DNA damage repair, cell cycle regulation, transcriptional regulation, and other important pathways to suppress tumor and maintain genome stability. A total of 120 Sahiwal cattle were selected to characterize the targeted region of intron 6 of BRCA1 gene for polymorphism screening and their association with mastitis. A 321bp PCR fragment of BRCA1 gene encompassing the targeted region of intron 6 was amplified and digested with Hha I to screen for the reported SNPs having significant association with SCS. Genotype analysis using PCR-RFLP revealed a monomorphic banding pattern. Sequencing was also carried out to explore the in silico screened SNPs which are deposited in dbSNP. The result indicates highly conserved sequence in Sahiwal cattle. Therefore, reported as well as in silico SNPs cannot be considered as a universal marker for mastitis in all the breeds. Since, present study has formulated the results based on a relatively small sample; further studies are required to screen these SNPs in large samples.
Keywords: Sahiwal, Polymorphism, BRCA1, Mastitis, PCR-RFLP.
Title: Characterization and Validation of Point Mutation in Breast Cancer 1 (BRCA1) and Its Relationship with Mastitis Traits in Sahiwal
Author: Ankit Magotra, I.D.Gupta, Archana Verma, Rani Alex, Vijay Kumar, M.R. Vineeth, Ashwani Arya, A. Sakthivel Selvan
ISSN 2350-1049
International Journal of Recent Research in Interdisciplinary Sciences (IJRRIS)
Paper Publications